Journal: Annals of Hematology

Article Title: Antithrombin III is probably not a suitable biomarker for diagnosis of primary central nervous system lymphoma

doi: 10.1007/s00277-015-2334-y

Figure Lengend Snippet: Cerebrospinal fluid (CSF) and serum antithrombin III (AT III) and albumin levels

Article Snippet: Serum was prepared from blood by centrifugation at 1500 × g for 10 min. AT III concentrations were measured by ELISA (Human Antithrombin III ELISA kit, cat. no. KSP-111, Nordic BioSite, Täby, Sweden) according to the manufacturer’s instructions.

Techniques:

Journal: Annals of Hematology

Article Title: Antithrombin III is probably not a suitable biomarker for diagnosis of primary central nervous system lymphoma

doi: 10.1007/s00277-015-2334-y

Figure Lengend Snippet: Cerebrospinal fluid (CSF) antithrombin III (AT III) and albumin concentrations and AT III/ albumin concentration ratio in different patient groups. a AT III concentrations in patients with primary CNS lymphoma (PCNSL), secondary CNSL (sCNSL), systemic lymphoma, and non-neoplastic disease as a control group of patients. b AT III concentrations in patients with CNS lymphoma (both PCNSL and sCNSL), multiple sclerosis (MS) disease, Alzheimer’s disease, and in control group patients. c Albumin concentrations in patients with PCNSL, sCNSL, systemic lymphoma without CNS lesion, and non-neoplastic disease as a control group of patients. d AT III/albumin ratio in patients with PCNSL, sCNSL, systemic lymphoma without CNS lesion, and non-neoplastic disease as a control group of patients

Article Snippet: Serum was prepared from blood by centrifugation at 1500 × g for 10 min. AT III concentrations were measured by ELISA (Human Antithrombin III ELISA kit, cat. no. KSP-111, Nordic BioSite, Täby, Sweden) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Control

Journal: Annals of Hematology

Article Title: Antithrombin III is probably not a suitable biomarker for diagnosis of primary central nervous system lymphoma

doi: 10.1007/s00277-015-2334-y

Figure Lengend Snippet: a Tumor cell antithrombin III (AT III) immunoreactivity reverse correlation with serum AT III concentrations. b Immunohistochemistry of lymphoma tissue, demonstrating tumor cell cytoplasmic expression of AT III and also AT III staining positivity around capillaries. Antithrombin III antibody staining, ×40 magnification. c Immunohistochemistry of lymphoma tissue, demonstrating strong expression of AT III in tumor cell cytoplasm and nuclear membrane. Antithrombin III antibody staining, ×100 magnification

Article Snippet: Serum was prepared from blood by centrifugation at 1500 × g for 10 min. AT III concentrations were measured by ELISA (Human Antithrombin III ELISA kit, cat. no. KSP-111, Nordic BioSite, Täby, Sweden) according to the manufacturer’s instructions.

Techniques: Immunohistochemistry, Expressing, Staining, Membrane

Journal: Annals of Hematology

Article Title: Antithrombin III is probably not a suitable biomarker for diagnosis of primary central nervous system lymphoma

doi: 10.1007/s00277-015-2334-y

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve analysis of CNS lymphoma patients, including both primary and secondary CNS lymphomas, versus patients with non-neoplastic neurological disorders to evaluate the possible role of antithrombin III (AT III) in cerebrospinal fluid (CSF) to be a novel biomarker to separate CNS lymphomas from other neurological disorders. 0-hypothesis was area under curve (AUC) 0.500, and values close to 1.000 are found significant and possibly useful as biomarkers in ROC analysis

Article Snippet: Serum was prepared from blood by centrifugation at 1500 × g for 10 min. AT III concentrations were measured by ELISA (Human Antithrombin III ELISA kit, cat. no. KSP-111, Nordic BioSite, Täby, Sweden) according to the manufacturer’s instructions.

Techniques: Biomarker Discovery

Journal: The Journal of Neuroscience

Article Title: Cloning and Functional Expression of an Aplysia 5-HT Receptor Negatively Coupled to Adenylate Cyclase

doi: 10.1523/JNEUROSCI.18-15-05586.1998

Figure Lengend Snippet: Affinities of various compounds that compete with the binding of 1.5 nM [ 3 H]LSD to the membranes of HEK 293 cells stably transfected with the 5-HT ap1 gene

Article Snippet: Alprenolol hydrochloride, clozapine, dopamine hydrochloride, (±)-8-hydroxy-2-(di- n -propyl-amino) tetralin hydrobromide (8-OH-DPAT), ketanserin tartrate, mesulergine hydrochloride, metergoline, methysergide maleate, NAN-190 hydrobromide, p -aminophenethyl- m -trifluoromethylphenyl piperazine, R -(+)-SCH-23390 hydrochloride, spiperone hydrochloride, and yohimbine hydrochloride were generous gifts from BioSignal Inc. (Montréal, Québec, Canada).

Techniques: Binding Assay, Stable Transfection, Transfection

Journal:

Article Title: Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis

doi: 10.1046/j.1365-2249.2003.02268.x

Figure Lengend Snippet: IL-10 (a), IL-2 (b), TGF-β1 (c), IFN-γ(d), IL-4 (e) and TNF-α(f) mRNA levels in freshly isolated colonic lamina propria T lymphocytes, CD3+ LPL, of ulcerative colitis patients (UC), patients with Crohn's disease (CD) and control patients (Ctrl). The amounts of cytokine mRNA and GAPDH mRNA were determined using the quantitative TaqMan EZ real time quantitative RT-PCR technology. The average level of cytokine mRNA/T cell was calculated as the ratio between the concentration of cytokine mRNA copies and GAPDH mRNA units in each sample. For the different cytokines each circle or triangle represents the value from a single subject. Filled circles indicate samples from patients with active colitis. Open circles indicate samples from patients with inactive colitis. Triangles indicate samples from controls. Horizontal bars indicate medians. Statistically significant differences with P-values ≤ 0·01 are indicated. (Mann–Whitney's ranking test).

Article Snippet: The anticytokine antibodies used in the study were: anti-IL-2 (clone 80-3418-01, mouse IgG1; Genzyme, Cambridge, MA, USA); anti-IL-4 (clone IL-4I(82), mouse IgG1), anti-IL-10 (clone JES3–9D7, rat IgG1, and clone JES3–19F1, rat IgG2a), anti-IFN- γ (clone 1-DIK, mouse IgG1) (all five from Mabtech, Nacka, Sweden); anti-IL-5 (clone TRFK5, rat IgG1), anti-IFN- γ (clone MMHG-1, mouse IgG1), TGF- β (clone TB21, mouse IgG1) (all three from Nordic Biosite, Täby, Sweden); anti-IL-4 (clone 25D2, rat IgG1; Endogen, Woburn, MA, USA); anti-TGF- β (IgG fraction of rabbit antiserum; R&D Systems, Abingdon, UK); anti-TNF- α (clone Mab1, mouse IgG1; Pharmingen, San Diego, CA, USA).

Techniques: Isolation, Control, Quantitative RT-PCR, Concentration Assay, MANN-WHITNEY

Journal:

Article Title: Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis

doi: 10.1046/j.1365-2249.2003.02268.x

Figure Lengend Snippet: Relative contribution of cytokine mRNA expressed by different lamina propria T lymphocyte subsets in UC and control colon

Article Snippet: The anticytokine antibodies used in the study were: anti-IL-2 (clone 80-3418-01, mouse IgG1; Genzyme, Cambridge, MA, USA); anti-IL-4 (clone IL-4I(82), mouse IgG1), anti-IL-10 (clone JES3–9D7, rat IgG1, and clone JES3–19F1, rat IgG2a), anti-IFN- γ (clone 1-DIK, mouse IgG1) (all five from Mabtech, Nacka, Sweden); anti-IL-5 (clone TRFK5, rat IgG1), anti-IFN- γ (clone MMHG-1, mouse IgG1), TGF- β (clone TB21, mouse IgG1) (all three from Nordic Biosite, Täby, Sweden); anti-IL-4 (clone 25D2, rat IgG1; Endogen, Woburn, MA, USA); anti-TGF- β (IgG fraction of rabbit antiserum; R&D Systems, Abingdon, UK); anti-TNF- α (clone Mab1, mouse IgG1; Pharmingen, San Diego, CA, USA).

Techniques: Control

Journal:

Article Title: Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis

doi: 10.1046/j.1365-2249.2003.02268.x

Figure Lengend Snippet: Frequency of cytokine positive cells in colonic tissue of ulcerative colitis (UC) and control patients as determined by immunomorphometry

Article Snippet: The anticytokine antibodies used in the study were: anti-IL-2 (clone 80-3418-01, mouse IgG1; Genzyme, Cambridge, MA, USA); anti-IL-4 (clone IL-4I(82), mouse IgG1), anti-IL-10 (clone JES3–9D7, rat IgG1, and clone JES3–19F1, rat IgG2a), anti-IFN- γ (clone 1-DIK, mouse IgG1) (all five from Mabtech, Nacka, Sweden); anti-IL-5 (clone TRFK5, rat IgG1), anti-IFN- γ (clone MMHG-1, mouse IgG1), TGF- β (clone TB21, mouse IgG1) (all three from Nordic Biosite, Täby, Sweden); anti-IL-4 (clone 25D2, rat IgG1; Endogen, Woburn, MA, USA); anti-TGF- β (IgG fraction of rabbit antiserum; R&D Systems, Abingdon, UK); anti-TNF- α (clone Mab1, mouse IgG1; Pharmingen, San Diego, CA, USA).

Techniques: Control

Journal:

Article Title: Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis

doi: 10.1046/j.1365-2249.2003.02268.x

Figure Lengend Snippet: Immunohistochemical staining for IL-2 (a, b), TGF-β (c, d) and IFN-γ (e, f) in the colonic mucosa of an UC patient (a, c, e) and a control patient (b, d, f); (a), (c) and (e) show sequential sections of UC. The bold arrow indicates an equivalent position in the sections. For abbreviations see Fig. 4. Counterstained with Mayer's haematoxylin. Original magnification ×51 in (a, b, c, d, e, f), and ×128 in the insert in (d).

Article Snippet: The anticytokine antibodies used in the study were: anti-IL-2 (clone 80-3418-01, mouse IgG1; Genzyme, Cambridge, MA, USA); anti-IL-4 (clone IL-4I(82), mouse IgG1), anti-IL-10 (clone JES3–9D7, rat IgG1, and clone JES3–19F1, rat IgG2a), anti-IFN- γ (clone 1-DIK, mouse IgG1) (all five from Mabtech, Nacka, Sweden); anti-IL-5 (clone TRFK5, rat IgG1), anti-IFN- γ (clone MMHG-1, mouse IgG1), TGF- β (clone TB21, mouse IgG1) (all three from Nordic Biosite, Täby, Sweden); anti-IL-4 (clone 25D2, rat IgG1; Endogen, Woburn, MA, USA); anti-TGF- β (IgG fraction of rabbit antiserum; R&D Systems, Abingdon, UK); anti-TNF- α (clone Mab1, mouse IgG1; Pharmingen, San Diego, CA, USA).

Techniques: Immunohistochemical staining, Staining, Control

Journal: The Journal of Reproduction and Development

Article Title: Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa

doi: 10.1262/jrd.2017-141

Figure Lengend Snippet: Detection of the CD44 receptor by Western blotting (WB) in human and pig spermatozoa. In A, using a porcine-specific anti-CD44 monoclonal antibody (60224-1-Ig, Nordic BioSite, Proteintech), the CD-44 receptor was detected in pig and human spermatozoa at 85 kDa. Gel B depicts co-incubation of this monoclonal primary antibody with its specific blocking peptide (Ag7633, Nordic BioSite, Proteintech Europe, Manchester, UK), which eliminated the 85 kDa band of the full-sized CD-44 receptor in all cells/tissues. L1: ladder, L2: human, L3: non-capacitated boar spermatozoa, L4: capacitated boar spermatozoa, L5: boar seminal vesicle (somatic positive control).

Article Snippet: The insets in the top-right corner in each figure (marked A´–C´) depict ICC negative control images (where the antibody was neutralized by treatment with the specific blocking peptide CD44 fusion protein Ag7633 (Nordic BioSite, Proteintech Europe, Manchester, UK)).

Techniques: Western Blot, Incubation, Blocking Assay, Positive Control

Journal: The Journal of Reproduction and Development

Article Title: Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa

doi: 10.1262/jrd.2017-141

Figure Lengend Snippet: Localization of the CD44 receptor on immunocytochemistry (ICC) analysis in human (A) and pig (B: uncapacitated, C: capacitated) spermatozoa using a monoclonal antibody (60224-1-Ig, Nordic BioSite, Proteintech Europe, Manchester, UK). The insets in the top-right corner in each figure (marked A´–C´) depict ICC negative control images (where the antibody was neutralized by treatment with the specific blocking peptide CD44 fusion protein Ag7633 (Nordic BioSite, Proteintech Europe, Manchester, UK)). Human spermatozoa (positive control) had clear CD44-immunostaining over the post-acrosome area of the sperm head (Fig. 2A). In pig spermatozoa (Fig. 2B; 2C), the CD44 receptor appeared consistently localized on the plasma membrane over the post-acrosomal region, neck, and midpiece. Confocal laser scanning microscopy, scale bar: 10 μm.

Article Snippet: The insets in the top-right corner in each figure (marked A´–C´) depict ICC negative control images (where the antibody was neutralized by treatment with the specific blocking peptide CD44 fusion protein Ag7633 (Nordic BioSite, Proteintech Europe, Manchester, UK)).

Techniques: Immunocytochemistry, Negative Control, Blocking Assay, Positive Control, Immunostaining, Confocal Laser Scanning Microscopy